Activation of recF-dependent recombination in Escherichia coli by bacteriophage lambda- and P22-encoded functions
نویسندگان
چکیده
منابع مشابه
Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli.
Replacement of Escherichia coli's RecBCD function with phage lambda's Red function generates a strain whose chromosome recombines with short linear DNA fragments at a greatly elevated rate. The rate is at least 70-fold higher than that exhibited by a recBC sbcBC or recD strain. The value of the system is highlighted by gene replacement with a PCR-generated DNA fragment. The deltarecBCD::Plac-re...
متن کاملProgress toward a metabolic interpretation of genetic recombination of Escherichia coli and bacteriophage lambda.
is a metabolically active cellular component of bacteria. TranscripDNA tion and replication can proceed continuously throughout the growth and division of bacterial cells. Enzymes methylate DNA bases and when they have not acted restriction enzymes nick and break double-stranded DNA molecules producing substrates for further degradation. When radiations or chemicals damage DNA, repair enzymes e...
متن کاملGenetic recombination in bacteriophage lambda
© The Author 2012. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Genetic recombination in bacteriop...
متن کاملIdentification of Bacteriophage-Encoded Anti-sRNAs in Pathogenic Escherichia coli
In bacteria, Hfq is a core RNA chaperone that catalyzes the interaction of mRNAs with regulatory small RNAs (sRNAs). To determine in vivo RNA sequence requirements for Hfq interactions, and to study riboregulation in a bacterial pathogen, Hfq was UV crosslinked to RNAs in enterohemorrhagic Escherichia coli (EHEC). Hfq bound repeated trinucleotide motifs of A-R-N (A-A/G-any nucleotide) often ass...
متن کاملIsolation of the bacteriophage lambda receptor from Escherichia coli.
A factor which inactivates the phage lambda can be extracted from Escherichia coli. This factor is a protein and is located in the outer membrane of the bacterial envelope. It is found in extracts of strains which are sensitive to phage lambda, but not in extracts of strains specifically resistant to this phage. We conclude that this factor is the lambda receptor, responsible for the specific a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1988
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.170.9.4379-4381.1988